Take the Aǹalemma out of the case and immerse the tube in the room temperature water. If you are using a regular glass (8-10 ounces), swirl the Aǹalemma through the water for at least 15 seconds. If you are using a water jug of 35 or more ounces, swirl the Aǹalemma for at least 30-40 seconds.
After use, wipe off the water drops from the Aǹalemma (with a cloth or a paper towel) and put it back in its case.
You can use the ratios above to calculate the time of swirling for any larger or smaller quantity of water.